Haemophilus transformation check results

 

 

 

 

Last week I did some transformation assays to double check some mutants that we have conflicting data for. I did three different sets of experiments. Notable findings: LigA had a reduced transformation frequency when we expected normal transformation, and for RR3116 I expected a ten fold decrease in transformation but had too many colonies to count on those plates . Everything else lined up with expectations

Raw Data

K  = 9.42E-04 expected: normal

comF = 1.63E-03 expected: normal

 

K2 = 1.27E-03 expected: normal

ligA 9.03E-05 expected: normal

pilF2 7.20E-09 expected: No transformants

 

K2 7.26E-04 expected: normal

comJ 7.86E-04 expected: normal

RR3123 1.79E-03 expected: normal

RR3116 Too many to count expected: 10 fold down

comP 7.69E-09 expected: No transformants

 

 

 

 

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2 Comments (+add yours?)

  1. Rosie Redfield
    Jun 15, 2011 @ 21:37:43

    Is ‘too many to count’ too many to estimate?

    Reply

    • dnogas
      Jun 16, 2011 @ 11:55:22

      Too many to estimate. There were bald spots on all but one of the Nov plates and more colonies than I would have expected if the transformatility was wild type, so maybe human error(maybe I plated a 10^-1 dilution instead of 10^-2?). The strain needs to be rechecked I don’t feel confident making any inferences from my data

      Reply

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