MurE information from the Ma and Redfield paper.

What we know about MurE

– Log phase growth is the unaffected in MurE mutants. The ability to recover from stationary growth is unchanged. No difference in sensitivity to osmotic shock. No differences in sensitivity to Gentamycin indicating no change to cell envelope permability. No differences in aztreonam(septation protein PBP3 inhibitor) or imipenem and mecillinam(PBP2 inhibitors) sensitivity

– MurE mutants do not constitutively take up DNA

– MurE mutants have the same specificity to the uptake signal sequence as wild type

– Mutations in essential competence genes(cya, crp, sxy, rec-2, comE, dprA?) prevent transformation in murE mutants as well. This paper is a little outdated, dprA isn’t an essential gene, its transformation frequency is just close to limit of detection

– LacZ fusions show greatly increased expression of comA and rec-2 in murE mutants, and slightly decreased sxy expression. Might be outdated data I need to check out the microarrays when I get back to the lab

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2 Comments (+add yours?)

  1. Rosie Redfield
    May 23, 2011 @ 10:34:48

    MurE mutants do not constitutively take up DNA? Perhaps this depends on how strictly we define ‘constitutively’. Do they take up DNA in proportion to how much they transform?

    Reply

    • dnogas
      May 23, 2011 @ 12:12:39

      I’m not sure, that’s a good idea. I think the idea behind constituitve is that uptake increases under certain conditions, not that it’s ever zero under any conditions. I’d also like to test if theres a different ratio of DNA in the periplasm vs cytosol. I’m doing the nucleotide safety training this week so I’ll be able to do those assays soon

      Reply

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