Plate observation

The colonies that pop up on transformed/antibiotic plates are very heterogenous in size. If delayed growth was a result of some property of the plate(either high cell denisty or antibiotic) ten you would expect more homogenous growth. This suggests delayed growth is a property of the cells themselves, and not plate conditions

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2 Comments (+add yours?)

  1. Clem
    Apr 04, 2011 @ 05:25:31

    Are all these colonies of clonal origin – or are they transformants? If the latter, is it possible the various colonies result from different transformants each with its own growth rate – due to the impact of the trasformation event on the rest of its genome?

    Reply

    • dnogas
      Apr 04, 2011 @ 11:22:07

      All transformants, but I have to do better controls. You got it! And it would make sense since every colony would start with a cell that had different transformation events – I transform with chromosomal DNA, all the colonies will pick up the NOV-r allele but also can/do take up random bits here and there. But it couldn’t just be a genetic change however, since the effect goes away over time – all the strains except for wildtype I plate on the plain plates are the result of earlier transformation events. I guess it could be selection but that seems very unlikely. Anouther possibility is that it’s not the transformation itself, but some more basal event that makes the cells competent that also makes them have this varied weird growth.

      Thanks for your interest and input/ideas/thoughts!

      Reply

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